Anti-virus software...
Anti-spyware software...
Heck, you can break out rootkit revealer:
http://technet.microsoft.com/en-us/sysinternals/bb897445.aspx But the only 100% effective thing to do is backup your data, wipe your machine, reload your operating system and programs and then put your data back. And then don't let anyone else touch your computer....
EDIT: did you start simple? Maybe she just has passwords to your email/facebook/myspace/whatever - change all your passwords to something random, put different ones on each site and see if that helps...